Identification of IgG and IgM in TB Patients During 2 Months of Treatment Using SDS-PAGE

Abstract

Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis. Several tests that can be performed to detect the presence of pulmonary TB are serological, one of which is, through the examination of immunoglobulin (Ig) G and M antibodies using the SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) method. This study aimed to determine whether IgG and IgM can be detected in TB patients during 2 months of treatment using SDS-PAGE. This study is descriptive by looking at the protein bands formed using SDS-PAGE. The results showed that all samples obtained IgM MW of 28.95 kDa; this protein's molecular weight (MW) indicates a humoral immune response in active TB patients. As for IgG, only the first sample obtained IgG MW of 157.97 kDa. This shows that the IgG MW, which is greater than the IgM MW, is formed after the patient has been on treatment for 2 months after experiencing TB infection and this IgG MW measure can last a long time even though the patient has recovered. Based on the results of this study, it is concluded that IgG and IgM in TB patients who have been on treatment for 2 months could detect IgG and IgM antibodies using SDS-Page. Thus, it is suggested for further researchers that the results of this study can be used as data and compared with TB patients who had 1 time exposed and 2 times exposed.